Nucleic Acid Purification

Nucleic acid purification involves extraction and selective isolation of nucleic acids such as DNA and RNA from various sources. Initially, the extraction process involves disrupting the cell membrane using salts, detergents or other methods. After this, the soluble and insoluble components of the cell lysate are separated, usually by centrifugation and vacuum filtration. Finally, the nucleic acids have to be separated from the rest of the soluble cell components, including proteins.

Various purification methods have developed over time. Some of the methods used are phenol-chloroform extraction, RNA extraction by Guanidinium thiocyanate-phenol-chloroform extraction, Spin column-based nucleic acid purification, Boom extraction method using silica beads.Automated processes provide easier and faster methods and additionally, often provide superior results and higher yield when compared to manual methods.

These purification processes require a variety of reagent solutions, kits, and other requirements. These cover DNA and RNA purification kits from sources of soil, water, bacteria, fungi, food products, animals and plants, cDNA extraction kits, wash buffers, guanidine thiocyanate reagent, GeneJet, and MagJet kits etc. Biomall can source and help you in acquiring your desired products from an array of selection, from popular brands such as Finnzymed, HiMedia, Fisher BioReagents, Pierce and many more.