Polymerase chain reaction or PCR is a technique which amplifies or makes multiple copies of a particular sequence of DNA in interest. It initially involves the denaturation of the double-stranded DNA at a temperature of 94 to 96C. This is followed by the annealing or joining of the specifically designed primer at the target site and finally, polymerase action leading to its elongation by the addition of the nucleotides. There are several variations of PCR including gradient PCR, digital PCR, Quantitative PCR (qPCR), Real Time-PCR, RT-PCR, nested PCR, multiplex PCR and so on.

This molecular biology tool requires a variety of reagent solutions and other requirements. These encompass buffer solutions, magnesium chloride solutions, SYBR green kits, genotype panels, polymerase kits, molecular biology grade water, primers, the sample DNA template, the deoxynucleoside triphosphates or dNTPs mixture, RNase inhibitors, RAPD kits etc. PCR Kits are available bundled with various products which are quick to use. Biomall canhelp you in acquiring your PCR Buffers, Reagents,and Kits from an array of selection, available from popular brands such as Finnzymes, HiMedia, Bangalore GeNei, Fisher BioReagents, ABgene and many more.