RNA Purifications and Extractions
RNA purification and extraction is the process of isolating RNA samples from biological extracts. There are many procedures for RNA extraction, the most common of which is Guanidinium thiocyanate-phenol-chloroform extraction. This is aliquidliquidextraction method which employs Guanidinium thiocyanate to denature proteins present in the sample. RNA partitions into the aqueous phase of the mixture. The phases are produced by phenol and chloroform in the solution.
However, the process is complicated by the universal presence of ribonuclease enzymes in cells and tissues, which rapidly degrade RNA. For this reason, equipment used in RNA extraction procedures is thoroughly cleaned and treated with harsh chemicals to destroy RNases. Another method of RNA extraction in liquid nitrogen, using a mortar and pestle (or tissue pulverizers) is also efficient in preventing ribonuclease activity.
Based on the requirements of researchers, various products are available. These include guanidine thiocyanate reagents, yeast RNA purification kits, plant RNA extraction kits etc., available from brands such as Fisher BioReagents, Finnzymes, HiMedia on Biomall.in
Aura Pure Bacterial RNA Isolation Kit, 250 Rxns
- Brand:
Aura Biotech - Catalog No.:
ABT-144L - Quantity/Unit: 250 Reactions/Pack
- Availability: In stock
Aura Pure Fungal/Plant RNA Isolation Kit, 50 Rxns
- Brand:
Aura Biotech - Catalog No.:
ABT-145S - Quantity/Unit: 50 Reactions/Pack
- Availability: In stock
Aura Pure Fungal/Plant RNA Isolation Kit, 100 Rxns
- Brand:
Aura Biotech - Catalog No.:
ABT-145M - Quantity/Unit: 100 Reactions/Pack
- Availability: In stock
Aura Pure Fungal/Plant RNA Isolation Kit, 250 Rxns
- Brand:
Aura Biotech - Catalog No.:
ABT-145L - Quantity/Unit: 250 Reactions/Pack
- Availability: In stock
Aura Pure Virus RNA Isolation Kit, 50 Rxns
- Brand:
Aura Biotech - Catalog No.:
ABT-155S - Quantity/Unit: 50 Reactions/Pack
- Availability: In stock
Aura Pure Virus RNA Isolation Kit, 100 Rxns
- Brand:
Aura Biotech - Catalog No.:
ABT-155M - Quantity/Unit: 100 Reactions/Pack
- Availability: In stock
Aura Pure Virus RNA Isolation Kit, 500 Rxns
- Brand:
Aura Biotech - Catalog No.:
ABT-155L - Quantity/Unit: 500 Reactions/Pack
- Availability: In stock
Water -2151100011730
- Brand:
Bangalore GeNei - Catalog No.:
2151100011730 - Quantity/Unit: 5 X 100 ml/Kit
- Availability: In stock
Water -2151181001730
- Brand:
Bangalore GeNei - Catalog No.:
2151181001730 - Quantity/Unit: 100 ml/Bottle
- Availability: In stock
10X TE -2151381001730
- Brand:
Bangalore GeNei - Catalog No.:
2151381001730 - Quantity/Unit: 100 ml/Bottle
- Availability: In stock
GeNei Total RNA Extraction Teaching Kit-6113200011730
- Brand:
Bangalore GeNei - Catalog No.:
6113200011730 - Quantity/Unit: 10 expts./Kit
- Usually Shipped in: 4-7 Days
RaFlex Total RNA Isolation Kit-2115100021730
- Brand:
Bangalore GeNei - Catalog No.:
2115100021730 - Quantity/Unit: 50 preps/Kit
- Availability: In stock
RaFlex Total RNA Isolation Kit -2115100031730
- Brand:
Bangalore GeNei - Catalog No.:
2115100031730 - Quantity/Unit: 50 preps/Kit
- Availability: In stock
GeNei Plant RNA Isolation Kit-2109800011730
- Brand:
Bangalore GeNei - Catalog No.:
2109800011730 - Quantity/Unit: 10 preps/Kit
- Availability: In stock
GeNei TRIsoln-2106481001730
- Brand:
Bangalore GeNei - Catalog No.:
2106481001730 - Quantity/Unit: 100ml/Bottle
- Availability: In stock
GeneiPure Total RNA Isolation Kit-2117100021730
- Brand:
Bangalore GeNei - Catalog No.:
2117100021730 - Quantity/Unit: 20 preps/Kit
- Availability: In stock
GeneiPure Total RNA Isolation Kit-2117500021730
- Brand:
Bangalore GeNei - Catalog No.:
2117500021730 - Quantity/Unit: 20 preps/Kit
- Availability: In stock
0.5M EDTA -2151280501730
- Brand:
Bangalore GeNei - Catalog No.:
2151280501730 - Quantity/Unit: 50 ml/Bottle
- Usually Shipped in: 4-7 Days
Sodium Acetate 3M -2151481001730
- Brand:
Bangalore GeNei - Catalog No.:
2151481001730 - Quantity/Unit: 100 ml/Bottle
- Availability: In stock
MicroRNA Isolation Kit-2124500021730
- Brand:
Bangalore GeNei - Catalog No.:
2124500021730 - Quantity/Unit: 20 preps/Kit
- Availability: In stock
3M Potassium acetate, pH 5.5-3100180501730
- Brand:
Bangalore GeNei - Catalog No.:
3100180501730 - Quantity/Unit: 50 ml/Bottle
- Availability: In stock
Total RNA Isolation Kit- Yeast, 20 preps
- Brand:
Bangalore GeNei - Catalog No.:
2117400021730 - Quantity/Unit: 1 Ea/Pack
- Availability: In stock
GeneiPure Total RNA Isolation Mini Kit-Plants, 20 preps
- Brand:
Bangalore GeNei - Catalog No.:
2117200021730 - Quantity/Unit: 20 rxns/pack
- Availability: In stock
GeneiPure Total RNA Isolation Mini Kit-Plants, 50 preps
- Brand:
Bangalore GeNei - Catalog No.:
2117200031730 - Quantity/Unit: 50 rxns/pack
- Availability: In stock
RNA loading dye, 1 mL
- Brand:
TEKBIO - Catalog No.:
TRLD100 - Quantity/Unit: 1mL/Vial
- Availability: In stock
Plant RNA Isolation kit, 50 Isolations
- Brand:
TEKBIO - Catalog No.:
TPRK50-Plant-Iso - Quantity/Unit: 50 Isolations/Box
- Availability: In stock
Plant RNA Extraction kit, 50 Extractions
- Brand:
TEKBIO - Catalog No.:
TPRK-50-Plant-Ext - Quantity/Unit: 50 Extractions/Box
- Availability: In stock
RNA Extraction kit-Plant-TPRK-50
- Brand:
Transiom Genomics - Catalog No.:
TPRK-50 - Quantity/Unit: 50 Extraction/Box
- Usually Shipped in: 1-2 Weeks