100bp DNA Ladder is supplied by HiMedia. This 100bp DNA ladder consists of 13 double stranded DNA fragments that range from 100 to 3,000 bp in size. The amounts of the 500 bp, 1,000 bp and 2,000 bp fragments are adjusted so that greater intensities of these three fragments can serve as easy references in an agarose gel. This product is supplied with a vial of 6X Gel loading buffer. A storage buffer consisting of 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 5% glycerol, 0.005% bromophenol blue and 0.005% xylene cyanol can be used to store this ladder. Some characteristics are:

Concentration: 100 g/ml

Recommended loading volume: 4 l/lane

Size range: 100 to 3,000 bp

DNA ladder or Gene ladder, consists of purified DNA bands spanning from 100 base pair to 1000 base pairs or even higher, depending on the size of the ladder. They are used to identify the molecular weight of an unknown linear fragment of the DNA when loaded and run in agarose gel electrophoresis, along with the test samples. A typical pack has 250 micrograms of the mass at 1 microgram/microliter concentration. Generally, one microliter is loaded for running on a single gel. The ladder is sensitive. A standard curve may be drawn to quantify DNA. It is advisable to store this product at -20C.