Laemmli Sample Buffer 10x is a premixed buffer for protein sample preparation prior to loading on SDS- PAGE. It is supplied as 10x concentrate. 20 l B-mercaptoethanol should be added per 80 l LaemmliSample Buffer 10x prior to use.Denser bands: It allows for thicker bands especially when working with a small number of cells, lysates with low concentration, and proteins with low expression. Ease of use: 10x Laemmli Sample Buffer is easily loaded into the wells thanks to its unique content. Quick: No loss of time with freeze and thaw as it is stored at room temperature.