10X Tris-Glycine Buffer, pH 8.3 is supplied by HiMedia. It is available in the form of a colourless solution with a pH in the range of 8.2 to 8.4. 10X Tris-Glycine Buffer, pH 8.3 is commonly used as a electrophoresis buffer in native polyacrylamide gel electrophoresis. This buffer is also used to prepare Tris-glycine-methanol transfer buffer during western blotting procedure. This buffer is supplied as a 10X stock and should be diluted to 1X before use. 10X Tris-Glycine Buffer, pH 8.3 is composed of 0.25 M Tris and 1.92 M Glycine and the pH is adjusted to 8.3. It is advisable to store this product at 15-25C.

10X Tris-Glycine Buffer, pH 8.3 is formulated for use in preparing gel electrophoresis buffer for native PAGE as this buffer does not contain SDS. This buffer is very convenient to use as it has to be only diluted to 1X with distilled water before performing electrophoresis. 10X Tris-Glycine Buffer is also used to prepare Tris-glycine methanol transfer buffer for blotting of protein samples on the nitrocellulose membranes during western blotting. This 10X concentrated buffer is diluted to 1X with methanol and water to make a solution containing 20% methanol for western transfer procedure. Methanol is required to prevent the gel from swelling during the transfer and enhances the binding of protein samples to nitrocellulose. 10X Tris-Glycine Buffer, pH 8.3 is used in native polyacrylamide gel electrophoresis of proteins and during the electro-transfer of protein samples from the polyacrylamide gels to the nitrocellulose membrane.