1M Tris Cl, pH 7.0, is supplied by HiMedia. It is available in the form of a colourless solution with a pH in the range of 6.9 to 7.1. Tris is the common name of tris (hydroxymethyl) aminomethane. It is a primary amine and is effective as a buffer in the pH range between 7.0 and 9.2. Tris does not precipitate calcium salts and maintains solubility of manganese salts. It has a low UV absorbance but strong temperature sensitivity. This buffer is supplied as 1M. It is advisable to store this product at room temperature (15-25C). It is composed of 1M Tris, pH adjusted to 7.0 with HCl. These solutions are ready for use and require no extra preparation.

1M Tris Cl, pH 7.0 is an extensively used buffer in Molecular Biology. It is also used as the major component of several buffer solutions, e.g. Tris-EDTA buffer, Tris-acetate EDTA buffer, Tris-borate EDTA buffer, Tris-buffered saline etc. Tris HCl is an organic compound which is highly soluble in water. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification. This product can be diluted to obtain the desired concentration.