The number of bands in this product is 8. The 50bp DNA ladder is ideal for determining the size of double-stranded DNA from 50 to 500 base pairs. The ladder consists of 8 linear double-stranded fragments .The 250bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 250bp are 40ng. The 250bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.