The number of bands in this product is 13. The 50bp ladder plus is ideal for determining the size of double-stranded DNA from 50 to 1000 base pairs. The ladder consists of 8 linear double-stranded fragments .The 250bp and 500bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 250bp and 500bp are 40ng. The 250bp and 500bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.