AK Agar No.2 (Sporulating Agar) (Arret and Kirshbaum Medium) is supplied by the company, HIMEDIA.

This is a sporulating agar named Arret and Kirshbaum Agar No.2 which is recommended for production of spores of Bacillus subtilis ATCC 6633 which is further used in the Penicillin Milk Test procedure. Due to the presence of yeast extract, HM peptone B, gelatin peptone and tryptone, source of vitamins and essential amino acids, this AK Agar No.2 medium is highly nutritious.

Product specifications:

• Catalog number:M234-500G
• Brand: HIMEDIA
• Quantity: Pack of 500g

Product Composition:gm/L

• Gelatin peptone: 6.000
• Tryptone: 4.000
• Yeast extract: 3.000
• HM peptone B #: 1.500
• Dextrose (Glucose): 1.000
• Manganous sulphate :0.300
• Agar: 15.000
• Final pH ( at 25C) :6.60.2

Productfeatures:

• Appearance: Cream to yellow homogeneous free flowing powder
• Gelling: Firm, comparable with 1.5% Agar gel
• Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates
• Reaction: Reaction of 3.08% w/v aqueous solution at 25C. pH : 6.60.2
• pH:6.40-6.80
• Cultural Response : Cultural characteristics observed after an incubation at 35-37C for 5 days.
• Storage and Shelf Life:Store between 10-30C in a tightly closed container and the prepared medium at 20-30C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.