APT Agar is supplied by the company, HIMEDIA.

This is an APT Agar recommended for cultivation and maintenance of Lactobacilli.This APT Agaris also used in the microbiological assay of thiamine. Thus, APT Agar can be used for cultivation of heterofermentative lactic acid bacteria requiring high thiamine content.

Product specifications:

• Catalog number:M226-500G
• Brand: HIMEDIA
• Quantity: Pack of 500g

Product Composition:gm/L

• Tryptone: 12.500
• Yeast extract: 7.500
• Dextrose (Glucose): 10.000
• Sodium citrate: 5.000
• Sodium chloride: 5.000
• Dipotassium hydrogen phosphate :5.000
• Magnesium sulphate :0.800
• Manganese chloride: 0.140
• Ferrous sulphate: 0.040
• Polysorbate 80 (Tween 80): 0.200
• Thiamine hydrochloride: 0.001
• Agar: 15.000
• Final pH ( at 25C) :6.70.2

Productfeatures:

• Appearance: Cream to yellow homogeneous free flowing powder
• Gelling: Firm, comparable with 1.5% Agar gel
• Colour and Clarity: Yellow coloured clear to slightly opalescent gel forms in Petri plates
• Reaction: Reaction of 6.12% w/v aqueous solution at 25C. pH : 6.70.2
• pH:6.50-6.90
• Cultural Response :Cultural characteristics observed after an incubation at 35-37C for 18-24 hours.
• Storage and Shelf Life: Store between 10-30C in a tightly closed container and the prepared medium at 20-30C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.