The number of bands in this product is 6. Marker 1 is ideal for determining the size of double-stranded DNA from 100 to 600 base pairs. The ladder consists of 6 linear double-stranded fragments. The 400bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 l loading, all fragments except 400bp are 50ng. The 400bp fragment is 125ng. This ladder is pre-mixed with loading dye and is ready to use.