Biuret Reagent is supplied by HiMedia. It is available in the form of a blue coloured solution.Biuret Reagent is an aqueous solution of potassium sodium tartrate treated with cupric sulfate and sodium hydroxide. In the presence of peptide bonds (protein), this blue solution will change to pink-purple colour.

Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques. Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate (HPTH), oyster hemolymph and Clostridium cellulovorans (CBDclos) aggregates.