Blood agar base no. 2 is supplied by the company, HIMEDIA.

This Blood agar base no. 2is is an excellent medium, when mixed with blood, allows the maximum recovery of fastidious microorganisms such as Streptococci, Pneumococci and other pathogens without interfering with their haemolytic reactions.

Product specifications:

• Catalog number:M834-100G
• Brand: HIMEDIA
• Quantity/unit: Pack of 100g

Product composition:gm/L

• Proteose peptone: 15.000
• HL extract : 2.500
• Yeast extract: 5.000
• Sodium chloride: 5.000
• Agar: 15.000
• Final pH ( at 25C): 7.40.2

Product features:

• Appearance: Cream to yellow homogeneous free flowing powder
• Gelling: Firm, comparable with 1.5% Agar gel
• Colour and Clarity of prepared medium: Basal medium : Light amber coloured clear to slightly opalescent gel. After addition of 5% v/v sterile defibrinated blood : Cherry red coloured opaque gel forms in Petri plates.
• Reaction: Reaction of 4.0% w/v aqueous solution at 25C. pH : 7.40.2
• pH: 7.20 - 7.60
• Cultural Response: Cultural characteristics observed with added 5% w/v sterile defibrinated blood,after an incubation at 35-37C for 18-48 hours.