Casitose, Type - I (Tryptone Type-1) is supplied by the company, HIMEDIA.

This Casitose, Type - I, also cited as Tryptone Type-1, is an enzymatic digest of milk protein, very rich in amino nitrogen that meets Pharmacopeial specification . This Casitose, Type - I is employed in the production of Sterility Testing Media such as Tryptone Soya Agar and broth, Fluid Thioglycollate Medium etc, and various Diagnostic Media. It is also used in media for fermentation processes to produce antibiotics and toxins. It is equivalent to Casein Enzyme Hydrolysate Type I.

Product specifications:

• Catalog number:RM014-500G
• Brand: HIMEDIA
• Quantity/unit: Pack of 500g

Product features:

• Appearance : Off white to light yellow homogenous free flowing powder ,having characteristic odour but not putrescent.
• Solubility: Freely soluble in distilled/purified water, insoluble in alcohol and ether. Clarity 2% w/v aqueous solution remains clear and neutral without any haziness after autoclaving at 15 lbs pressure (121C) for 15 minutes.
• Reaction: Reaction of 2% w/v aqueous solution at 25C.
• pH: 6.20- 7.20
• Test for Pathogens: 1. Escherichia coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosaNegative in 10 gms of sample 4. Staphylococcus aureus- Negative in 10 gms of sample5. Candida albicans- Negative in 10 gms of sample 6. Clostridia- Negative in 10 gms of sample
• Degree of digestion: As per method specified in USP 32, a. Absence of undigested protein b. Presence of proteoses c. Presence of tryptophan
• Nitrite test: As per method specified in USP 32 Negative: No development of pink or red colour.
• Microbial Content: As per method specified in USP 32 <=Total of 50 microorganisms or clumps in 10 consecutive fields.
• Bacteriological Testing: Bacteriological tests carried out as per USP 32 where respective medium is prepared by using Tryptone under test.
• Yeast & mould Count : <= 100 CFU/gram By plate method when incubated at 20-25C for not less than 5 days.
• Bacterial Count : <= 2000 CFU/gram By plate method when incubated at 30-35C for not less than 3 days.
• Microbial Load: Total Yeast and mould count (cfu/gm)
• Total aerobic microbial count (cfu/gm) Test for fermentable carbohydrate Medium :2% Tryptone Type-I (Casitose Type-I) w/phenol red broth w/durhams tube.After inoculation with test culture and incubation for 24 hours at 35-37C Escherichia coli ATCC 25922 No acid production or only trace in the inner tube,no gas production,(Positive test)
• Production of acetyl methyl carbinol Medium :1%Tryptone Type-I (Casitose Type-I) 0.5% of dextrose and 0.5% sodium chloride in water.After inoculation with test culture and incubation for 24 hours at 35-37C .