Chocolate No. 2 Agar Base is supplied by the company, HIMEDIA.

This is a Chocolate No. 2 Agar Base with supplements, employed for the cultural isolation of Neisseria and Haemophilus species from a variety of clinical specimens.

Product specifications:

• Catalog number:M1548-500G
• Brand: HIMEDIA
• Quantity/unit: Pack of 500g

Product composition:gm/L

• Casein enzymic hydrolysate :7.500
• Meat extract: 7.500
• Sodium chloride: 5.000
• Dipotassium phosphate :4.000
• Corn starch :1.000
• Monopotassium phosphate :1.000
• Agar :12.000
• Final pH ( at 25C): 7.30.2

Product features:

• Appearance: Cream to yellow homogeneous free flowing powder
• Gelling: Firm, comparable with 1.2% Agar gel
• Colour and Clarity of prepared medium :Basal medium: Light amber coloured clear to slightly opalescent gel. After addition of haemoglobin : Chocolate brown coloured opaque gel forms in Petri plates.
• Reaction :Reaction of 3.8% w/v aqueous solution at 25C.
• pH : 7.30.2
• Cultural Response M1548: Cultural characteristics observed with added 2% haemoglobin (FD022) and Vitamino Growth Supplement,Modified(FD215), after an incubation at 35-37C for 40-48 hours