CMRL 1066 Medium Without L-Glutamine, Phenol Red and Sodium bicarbonate is supplied by HiMedia. It is available in the form of an off-white to creamish white homogenous powder. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Magnesium sulphate anhydrous (97.69)

Potassium chloride (400.00)

Sodium acetate anhydrous (50.00)

Sodium chloride (6800.00)

Sodium dihydrogen phosphate anhydrous (122.00)

Amino acids

Choline chloride (0.50)

D-Biotin (0.01)

D-Pantothenate (Hemicalcium) (0.01)

Folic acid (0.01)

L-Ascorbic acid sodium salt (50.00)

Nicotinamide (0.025)

Nicotinic acid (0.025)

Pyridoxal hydrochloride (0.025)

Pyridoxine hydrochloride (0.025)

Riboflavin (0.01)

Thiamine hydrochloride (0.01)

myo-Inositol (0.05)

p-Amino benzoic acid (PABA) (0.05)

2' Deoxyadenosine 10.000

2' Deoxycytidine hydrochloride (10.00)

2' Deoxyguanosine (10.00)

5-Methyldeoxycytidine (0.10)

Cholesterol (0.20)

Cocarboxylase (1.00)

Coenzyme A sodium salt (2.50)

D-Glucose (1000.00)

D-Glucuronic acid sodium salt (3.88)

FAD disodium salt (0.11)

Glutathione (10.00)

Thymidine (10.00)

Tween 80 (5.00)

Uridine-5-Triphosphate.Na (1.00)

-NAD (7.00)

-NADP sodium salt (1.00)

CMRL media a chemically defined medium formulated for long term cultivation of mouse L cells in the absence of protein supplement. It is based on the composition of Medium 199 but the nucleic acid derivatives, coenzymes omitted in H597 modification are retained in CMRL 1066. Although originally designed for use in serum free conditions, this medium supports growth of a variety of cell lines in the presence of serum. It is advisable to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.