Combi 77- OD277R-1PK is 8-in-one multi disc, an octo disc, supplied by HiMedia. It contains an inert flat circular octa ring with 8 equidistant arms on the outer periphery, each arm having a 6mm disc. Each disc is satured with a specific antibiotic, that help in antibiotic susceptibility testing (AST) of Gram positive bacteria. Each antibiotic disc is about 24mm apart from the adjacent disc, and is labeled with the concentration and symbol of the respective antibiotic. OD277R octa rings can be used on 90 mm media plates. . The composition of antibiotics on the discs are as:

Amikacin (AK) 30g

Cefotaxime (CTX) 30g

Amoxycillin (AMX) 30g

Cefepime (CPM) 30g

Chloramphenicol (C) 30g

Ciprofloxacin (CIP) 5g

Isepamicin (IP) 30g

The Combi 77-OD277R rings are deposited aseptically in the center of the inoculated Mueller Hinton agar plate using sterile forceps. Immediately the plates are incubated at 352C for 16-18 hours. For fastidious organisms, incubate at appropriate temperature and time. The diameter of the zones of complete inhibition are measured to the nearest mm.

These multidiscs are convenient and economic for conducting AST. They give the priviledge to study large number of antibiotics at one time.

The Combi 77-OD277R discs should be stored between 2-8C under dry conditions and at -20C for prolonged use, along with the dessicator provided in indivisual pack.