Cooked Meat Medium is supplied by HiMedia. It is a sterile ready prepared media containing 10ml of clear solution in bottles. Cooked Meat Medium is generally used for the cultivation of aerobes and anaerobes, especially pathogenic Clostridia and also for the maintenance of stock cultures. It is advisable to store this ready prepared media between 2-8C. Composition (in gm/L) is as follows:

Beef heart, solids (98.00)

Proteose peptone (20.00)

Dextrose (2.00)

Sodium chloride (5.00)

Cooked Meat Medium was initially developed by Robertson for the cultivation of certain anaerobes isolated from wounds. The present formulation is a modification which supports the growth of many spore forming and non-spore forming strict anaerobes. This medium can initiate growth of bacteria from very small inoculum and maintain the viability of cultures over long period. Mixed cultures of bacteria survive in Cooked Meat Medium without displacing the slower-growing organisms. Cooked Meat Medium contains beef heart, as muscle protein, which provides amino acids and other nutrients. Beef heart also contains glutathione, a reducing substance that permits the growth of obligate anaerobes. The sulphydryl groups, which impart reducing effect, are more available in denatured protein and hence cooked meat is added in the medium. The addition of dextrose allows rapid and heavy growth of anaerobic bacteria in a short time and leads to more rapid identification of important anaerobes. Growth in this medium is indicated by turbidity or bubble formation by some organisms.