To be used in conjunction with Caymans CRTH2/DP2 Receptor (C-Term) polyclonal antibody (Catalog No. 10007002) to block protein-antibody complex formation during immunochemical analysis of CRTH2 Prostaglandin D2 (PGD2) is synthesized from PGH2 by hematopoietic- and lipocalin-type PGD synthases. PGD2 elicits its biological function through interaction with two distinct G protein-coupled receptors, DP1 and CRTH2/DP2. CRTH2 primarily couples to Gi or Gq subunits to mobilize Ca2+, induce cell migration, and up-regulate adhesion molecules.1 CRTH2 mRNA has been detected in various tissues including liver, lung, kidney, brain, hEArt, thymus, and spleen and in various cell linEAges including both hematopoietic and non-hematopoietic cell lines.2 Human CRTH2 is 395 amino acids in length with an estimated molecular weight of 43 kDa. Cayman Chemicals CRTH2/DP2 receptor (N-Term) polyclonal antibody can be used for western blot and immunohistochemical analysis of CRTH2 on samples of human, bovine, murine, and rat origin. It detects both unglycosylated and glycosylated protein at sizes ranging from 35-40 kDa to 50-70 kDa, as reported by Nagata et al., in 1999.3 Technical Information Synonyms Chemoattractant-receptor Homologous Molecule Expressed on Th2 Cells DP2 Receptor GPR44 Prostaglandin D2 Receptor 2 Amino Acids CRTH2/DP2 protein amino acids 378-395 Formulation 200 ug of peptide in 200 l TBS, pH 7.4, containing 0.1% BSA and 0.02% sodium azide