Diluent TSB with Cap 4 is supplied by HiMedia. It is a sterile ready prepared media containing 9ml of dark amber coloured clear solution in bottles. Diluent TSB with Cap 4 can be used for the detection and enumeration of microorganisms for products of sanitary importance, water miscible cosmetics, water insoluble products, fatty products containing antimicrobials or preservatives. It is advisable to store this ready prepared media between 2-8C. Composition (in gm/L) is as follows:

Pancreatic digest of casein (17.00)

Papaic digest of soyabean meal (3.00)

Sodium chloride (5.00)

Dextrose (2.50)

Dibasic potassium phosphate (2.50)

Cap 4 /100 ml (25.00ml)

Lecithin (2.00)

Peptone (1.00)

Tween 80 (14.00)

Tamol (3.00)

Distilled water (75.00ml)

Tryptone Soya Broth is suggested by various pharmacopeia as a sterility testing and as a microbial limit testing medium. This medium is a highly nutritious medium used for cultivation of a wide variety of organisms. The mixture of pancreatic digest of casein and papaic digest of soyabean meal makes the medium nutritious by supplying amino acids and long chain peptides for the growth of microorganisms. Dextrose and dibasic potassium phosphate provide carbohydrate and the buffer, respectively in the medium. Sodium chloride maintains the osmotic balance of the medium. Cap 4 added to the medium is a mixture of dispersant and neutralizers that are reported to disperse the hydrophobic copolymer and to inactivate residual disinfectants in the sample.