Dulbecco's Modified Eagle Medium (DMEM) With 4.5gm Glucose per litre, L-Glutamine, 25mM HEPES buffer, Sodium pyruvate and Sodium bicarbonate, is supplied by HiMedia. It is available in the form of an orangish red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Ferric nitrate nonahydrate (0.10)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (400.00)

Sodium bicarbonate (3700.00)

Sodium chloride (6400.00)

Sodium dihydrogen phosphate anhydrous (109.00)

Amino acids

Choline chloride (4.00)

D-Ca-Pantothenate (4.00)

Folic acid (4.00)

Nicotinamide (4.00)

Pyridoxal hydrochloride (4.00)

Riboflavin (0.40)

Thiamine hydrochloride (4.00)

i-Inositol (7.20)

D-Glucose (4500.00)

HEPES buffer (5958.00)

Phenol red sodium salt (15.90)

Sodium pyruvate (110.00)

Dulbecco's Modified Eagle Medium (DMEM) is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four-fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of Glucose and was originally used to culture embryonic mouse cells. DMEM high glucose is a further modification of original DMEM and contains 4500mg/L of glucose. The additional glucose has proved to be useful in cultivating various other cell lines including primary cultures of mouse and chicken cells as well as various normal and transformed cell lines. This is a 1X Liquid Cell Culture Medium with L-glutamine, 4.5gms glucose per litre, 25mM HEPES buffer, sodium bicarbonate and sodium pyruvate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.