Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) With L-Glutamine and 2.438gms per litre Sodium bicarbonate Without HEPES buffer and Trace elements, is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 320.00 -360.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.001)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Manganese sulphate (0.0001)

Nickel chloride (0.0001)

Potassium chloride (311.80)

Sodium bicarbonate (2438.00)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate (54.30)

Zinc sulphate heptahydrate (0.43)

Amino acids

Choline chloride (8.98)

D-Biotin (0.003)

D-Ca-Pantothenate (2.24)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.03)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic acid (0.10)

Hypoxanthine (2.40)

Linoleic acid (0.04)

Phenol red sodium salt (8.63)

Putrescine hydrochloride (0.08)

Sodium pyruvate (110.00)

Thymidine (0.36)

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This is a 1X Liquid Cell Culture Medium with L-glutamine and sodium bicarbonate, but without HEPES buffer. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.