Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/F12,1:1 mixture) With 15mM HEPES buffer, Sodium bicarbonate and Trace elements Without L-Glutamine, is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 300.00 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Ammonium metavanadate (0.0005)

Ammonium molybdate tetrahydrate (0.006)

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.001)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Manganese sulphate (0.0001)

Nickel chloride (0.0001)

Potassium chloride (311.80)

Sodium bicarbonate (1200.00)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate monohydrate (54.30)

Sodium metasillicate nonahydrate (0.014)

Sodium selenite (0.0051)

Stannous chloride dihydrate (0.0001)

Zinc sulphate heptahydrate (0.43)

Amino acids

Choline chloride (8.98)

D-Biotin (0.003)

D-Ca-Pantothenate (2.24)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.03)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic acid (0.10)

HEPES buffer (3574.50)

Hypoxanthine (2.40)

Linoleic acid (0.04)

Phenol red sodium salt (8.63)

Putrescine hydrochloride (0.08)

Sodium pyruvate (110.00)

Thymidine (0.36)

Dulbecco's Modified Eagle Medium / Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. This is a 1X Liquid Cell Culture Medium with sodium bicarbonate, 15mM HEPES buffer and trace elements. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium. It does not contain L-glutamine.