EMB BROTH is supplied by TM MEDIA. It is used for isolation of gram-negative enteric bacteria from clinical and non-clinical specimens. It is used to study of dierent enteric bacteria by adding dierent carbohydrates. The ratio of eosin and methylene blue is adjusted approximately to 6:1. Sucrose is added to the medium as an alternative carbohydrate source for typically lactose-fermenting, gram-negative bacilli, which on occasion do not ferment lactose or do so slowly. The coliforms produce purplish black colonies due to taking up of methylene blue-eosin dye complex, when the pH drops. The dye complex is absorbed into the colony. Nonfermenters probably raise the pH of surrounding medium by oxidative deamination of protein, which solubilizes the methylene blue-eosin complex resulting in colourless colonies.

Product features:-

• Appearance - Light pink to purple homogeneous free flowing powder
• Gelling -Firm, comparable with 1.35% Agar gel.
• Colour and Clarity of prepared medium - Reddish purple coloured, opalescent gel with greenish cast and finely dispersed precipitate forms in Petri plates
• Shelf life - 3 years
• Storage temperature - Below 30 deg C

Product specifications:-

• Product Name -EMB BROTH
• Brand Name - TM MEDIA
• Quantity - 500 gm
• Catalogue Number - TM314