K1612 is referred as First Strand cDNA Synthesis Kit under Thermo Fisher Scientific catalogue. K1612 First Strand cDNA Synthesis Kit is applicable in First strand cDNA synthesis for RT-PCR and real-time RT-PCR,Construction of cDNA libraries, Generation of probes for hybridization, and aRNA synthesis

. K1612 First Strand cDNA Synthesis Kit uses the recombinant M-MuLV Reverse Transcriptase which shows lower RNase H activity than AMV reverse transcriptase. Using this kit full-length cDNA can be synthesized from RNA templates up to 9 kb. The kit includes recombinant RiboLock RNase Inhibitor which effectively protects RNA template from degradation. The kit includes

M-MuLV Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, 10 mM each, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, Forward GAPDH Primer, 10 M (5'-CAAGGTCATCCATGACAACTTTG-3')

, Reverse GAPDH Primer, 10 M (5'-GTCCACCACCCTGTTGCTGTAG-3')and nuclease free water .

Specifications: K1612 First Strand cDNA Synthesis Kit should be stored at -20C. Optimal reaction temperature is 37 C. The product size is 100 reactions.