GeNei Competent Cells Preparation Kit (B) is a kit provided for convenient preparation of competent cells. Competent cells are those bacterial cells which are treated to be more ready to incorporated foreign DNA. Competent cells are used for transformation and further, for cloning. However, it is often seen that competent cell preparation is quite tricky, time consuming and yields poor transformation efficiency. The GeNei Competent Cells Preparation Kit (B) allows a user to generate competent cells with increased transformation efficiency. The kit promotes faster and cheaper preparation of competent cells. Under ideal reaction conditions and using E.coli DH5 and pUC 19 plasmid, the kit gives a high transformation efficiency of 5 * 105 106 transformants/g. The kit uses a modified calcium chloride method of competent cell preparation and all the necessary reagents are provided in the kit itself. The reagents are supplied in a pre-sterilized manner and the kit provides for 50 transformation reactions, wherein, each reaction would require 100 L of competent cells. The competent cells can be stored for later use at -70C.

The following materials are provided with the kit:

(e) Competent cell preparation solution (to be stored at 4 C)

(f) Glycerol (to be stored at 4 C)

(g) Components for bacterial growth media preparation:

- Yeast extract

- Tryptone

- Agar

- Sodium chloride

(h) Instruction manual for further reference

(i) Magnesium chloride

(j) Magnesium sulphate

(k) Potassium chloride

Product specifications:

Product name: GeNei Competent Cells Preparation Kit (B)

Catalogue number: 1660200011730

Brand: Genei

Number of experiments supported: 50 reactions

Price: 5,820.00