GeNei Hydrophobic Chromatography Teaching Kit, 5 expts is a product from Genei. Chromatography is a technique which is used to separate a mixture into its various components based on its differential mobility or attraction to another substrate. Hydrophobic chromatography is a special type of chromatography which uses hydrophobic interactions to purify or separate proteins. The GeNei Hydrophobic Chromatography Teaching Kit, 5 expts is upplied for easy demonstration and teaching of the principles of the technique to students. It has the following features and components:

- Column Matrix: The stationary phase contains the HIC column.

- Bacterial lysate sample: This is the sample which contains GFP, a highly hydrophobic protein because of the presence of a few hydrophobic amino acid groups.

When the bacterial lysate is passed through the column, the GFP, owing to its hydrophobicity, binds to the HIC and the other proteins simply pass through the column.

- Binding buffer: This buffer provides the appropriate binding environment for the GFP to the HIC.

- Equilibration buffer:

- Elution buffer: This is a high salt buffer which breaks the hydrophobic interactions between GFP and the HIC, thus allowing the GFP to elute.

- Storage buffer

- Instruction manual for further reference.

Product specifications:

Product name: GeNei Hydrophobic Chromatography Teaching Kit, 5 expts

Brand: Genei

Catalogue number: 6120200011730

PI number: KT202