Glasgow's Minimum Essential Medium(GMEM) With Sodium bicarbonate and Tryptose Phosphate Broth Without L-Glutamine, is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 310.00 -350.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (265.00)

Ferric nitrate nonahydrate (0.10)

Magnesium sulphate anhydrous (97.720)

Potassium chloride (400.00)

Sodium bicarbonate (2750.00)

Sodium chloride (6400.00)

Sodium dihydrogen phosphate, anhydrous (109.00)

Amino acids

Choline chloride (2.00)

D-Ca-Pantothenate (2.00)

Folic acid (2.00)

Nicotinamide (2.00)

Pyridoxal hydrochloride (2.00)

Riboflavin (0.20)

Thiamine hydrochloride (2.00)

i-Inositol (3.60)

D-Glucose (4500.00)

Phenol red sodium salt (15.00)

Tryptose Phosphate broth (2950.00)

Glasgow's Minimum Essential Medium (GMEM) is a modification of Basal Medium Eagle (BME). Ian Macpherson and Michael Stoker added tryptose phosphate broth and twice the concentration of amino acids and vitamins to BME. The medium was originally used to culture BHK-21 clone 13 cells, used for investigating the genetic factors affecting cell competence. This is a 1X Liquid Cell Culture Medium with sodium bicarbonate and tryptose phosphate broth. It does not contain L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.