High Range DNA Ladder is supplied by HiMedia. The High Range DNA ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 14 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-25kb base pairs. The 1kb and 3kb bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.52 g a load) for approximating the mass of DNA in comparably intense samples of similar size. This ladder is pre-mixed with loading dye and is ready to use. It is advisable to store this DNA ladder at -20C. Recommended load: 5 l / lane.

DNA ladder or Gene ladder, consists of purified DNA bands spanning from 100 base pair to 1000 base pairs or even higher, depending on the size of the ladder. They are used to identify the molecular weight of an unknown linear fragment of the DNA when loaded and run in agarose gel electrophoresis, along with the test samples. A typical pack has 250 micrograms of the mass at 1 microgram/microliter concentration. Generally, one microliter is loaded for running on a single gel. The ladder is sensitive. A standard curve may be drawn to quantify DNA.