HiGlutaXL Dulbeccos Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12,1:1 Mixture) With L-Alanyl-L-Glutamine, Sodium bicarbonate, 15mM HEPES buffer and Trace elements, is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 300.00 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Ammonium metavanadate (0.0005)

Ammonium molybdate tetrahydrate (0.006)

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.001)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Manganese sulphate (0.0001)

Nickel chloride (0.0001)

Potassium chloride (311.80)

Sodium bicarbonate (1200.00)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate monohydrate (54.30)

Sodium metasillicate nonahydrate (0.014)

Sodium selenite (0.0051)

Stannous chloride dihydrate (0.0001)

Zinc sulphate heptahydrate (0.43)

Amino acids

Choline chloride (8.98)

D-Biotin (0.003)

D-Ca-Pantothenate (2.24)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.03)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic acid (0.10)

HEPES buffer (3574.50)

Hypoxanthine (2.40)

Linoleic acid (0.04)

Phenol red sodium salt (8.63)

Putrescine hydrochloride (0.08)

Sodium pyruvate (110.00)

Thymidine (0.36)

HiGlutaXL medium contains the stabilized dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build-up of ammonia. L-alanyl-L-glutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of L-glutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4C for longer periods. This is a 1X Liquid Cell Culture Medium with L-alanyl-L-glutamine, sodium bicarbonate, 15mM HEPES buffer and trace elements. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.