HiGlutaXL Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 mixture) With L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements Without HEPES buffer, is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 290.00 -330.00 Osm/Kg H20. It is advisable to store this product between 2-8C. Media composition (in mg/L) is as follows:

Ammonium metavanadate (0.0005)

Ammonium molybdate tetrahydrate (0.006)

Calcium chloride dihydrate (154.50)

Copper sulphate pentahydrate (0.001)

Disodium hydrogen phosphate (71.02)

Ferric nitrate nonahydrate (0.05)

Ferrous sulphate heptahydrate (0.42)

Magnesium chloride hexahydrate (61.20)

Magnesium sulphate anhydrous (48.84)

Manganese sulphate (0.0001)

Nickel chloride (0.0001)

Potassium chloride (311.80)

Sodium bicarbonate (1200.00)

Sodium chloride (6996.00)

Sodium dihydrogen phosphate (54.30)

Sodium metasillicate nonahydrate (0.014)

Sodium selenite (0.0051)

Stannous chloride dihydrate (0.0001)

Zinc sulphate heptahydrate (0.43)

Amino acids

Choline chloride (8.98)

D-Biotin (0.003)

D-Ca-Pantothenate (2.24)

Folic acid (2.66)

Niacinamide (2.02)

Pyridoxal hydrochloride (2.00)

Pyridoxine hydrochloride (0.03)

Riboflavin (0.22)

Thiamine hydrochloride (2.17)

Vitamin B12 (0.68)

myo-Inositol (12.60)

D-Glucose (3151.00)

DL-Thioctic acid (0.10)

Hypoxanthine (2.40)

Linoleic acid (0.04)

Phenol red sodium salt (8.63)

Putrescine hydrochloride (0.08)

Sodium pyruvate (110.00)

Thymidine (0.36)

HiGlutaXL medium contains the stabilised dipeptide form of L-glutamine, L-alanyl-L-glutamine. HiGlutaXL medium offers several advantages over the conventional glutamine containing media. Dipeptide form prevents the intramolecular cyclization reaction, thus preventing toxic build -up of ammonia. L-alanyl-L-glutamine incorporates L-alanine that protects the alpha amino acid group. Aminopeptidases within the cell break the dipeptide, gradually releasing both L-glutamine and L-alanine for use by the cell. The gradual release of L-glutamine obviates the need to supplement L-glutamine frequently and allows liquid media to be stored at 4C for longer periods. This is a 1X Liquid Cell Culture Medium with L-alanyl-L-glutamine, sodium bicarbonate and trace elements. It does not contain HEPES buffer. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.