HiKaryoXLTM RPMI Medium is supplied by HiMedia. It is available in the form of an orangish red coloured clear solution which has a pH in the range of 7.0 to 7.6 and an osmolality between 340.00-380.00 Osm/Kg H20. HiKaryoXLTM RPMI Medium is composed of a basal medium RPMI 1640 and supplemented with L-Glutamine, FBS, PHA-P, Penicillin, Streptomycin and Sodium bicarbonate. It is a complete medium and does not require supplementation with any additional components. It is advisable to store this product at -20C, but not in a self-defrosting freezer. The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by counting the metaphases.

Quantity provided: 5 x 100ml /EA

HiKaryoXLTM RPMI Medium is a karyotyping medium recommended for short term in vitro culture of peripheral blood lymphocytes for cytogenetic studies. Cytogenetic studies include metaphase and pro-metaphase studies carried out on lymphocytes to detect chromosomal aberrations associated with structural and numerical abnormalities. Lymphocytes come from normal peripheral blood and are mitotically inactive, hence have to be stimulated with a mitogen such as Phytohemagglutinin M (PHA-M) or Phytohemagglutinin P (PHA-P). Phytohemagglutinin is a lectin extract from red kidney bean (Phaseolus vulgaris). PHA-P consists of two subunits, a leucoagglutinin (PHA-L) and an erythroagglutinin (PHA-E). PHA-P is a stronger blood agglutinin than PHA-M because of higher PHA-E (erythroagglutinin) activity. Hence, blood coagulation is commonly observed in media containing PHA-P, but it does not interfere with the karyotyping results in any way.