HiPurA Bacterial RNA Purification Kit is supplied by HiMedia. HiPurA Bacterial Purification Kit provides a fast and easy method for purification of Bacterial RNA for Northern analysis, Poly A+ RNA selection, Primer extension, RNase and S1 nuclease protection assays, RT-PCR, Differential display, Expression-array and expression-chip analysis and cDNA library construction. The RNA purification procedure using the miniprep spin columns comprises of three steps viz. adsorption of RNA to the membrane, removal of residual contaminants and elution of pure RNA. HiMedias HiElute Miniprep Spin column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality RNA is obtained from various species. The RNA obtained is compatible with various downstream applications as mentioned. This kit contains:

Lysozyme

RNA Lysis Solution (HRL)

Prewash Solution (RW1)

Wash Solution Concentrate (WS)

Elution Solution (RNase- Free Water)

HiElute Miniprep Spin Column (Capped)

HiShredder

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit makes isolation of RNA from different types of bacteria (Gram positive and Gram negative) easier by using a spin-column procedure. The lysis buffer enclosed, helps in cell disruption and denaturation. The samples can be centrifuged through a HiShredder which removes insoluble material and reduces the viscosity of the lysate by disrupting viscous material. Ethanol, when added to the cleared lysate, which promotes selective binding of RNA to the HiElute Miniprep Spin column membrane. After the initial binding of RNA,short washing steps can be used to remove impurities like proteins, polysaccharides, low molecular weight metabolites and salts. High quality RNA is finally eluted in the Elution Solution included in the\ kit.