HiPurA Banana DNA Isolation Kit is supplied by HiMedia. DNA extraction from plant tissues, unlike DNA isolation from mammalian tissues, remains difficult due to the presence of a rigid cell wall surrounding the plant cells. This method can be used both on freeze dried leaves and on fresh leaves. The scale of extraction is dependent on the amount of starting material, for e.g. 200 mg of material requires 900 l of Extraction Buffer and yields 10-50 g of DNA. The kit contains:

Banana Extraction Buffer

Additive-II

Additive-III

RNase A Solution (20 mg/ml)

Banana Wash Buffer Concentrate (WB1)

Precipitation Buffer (BS)

Elution Buffer (EB) [10mM Tris-Cl, 1.0mM EDTA, pH 8.5]

Collection Tubes, Polypropylene (2.0 ml)

Banana Extraction Buffer contains CTAB (Cetyltrimethylammonium bromide), a detergent used to break open plant cells and solubilize the contents. Chlorophyll and some denatured proteins are removed from green plant tissue in an organic chloroform-isoamylalcohol step, and the organic phase is separated by centrifugation. Since the extract contains DNA and RNA, RNA can be removed by the addition of RNase A, the DNA is precipitated and washed in organic solvents before re-dissolving in aqueous solution. This method can be used to process large number of samples on a daily basis. Very high DNA yield is obtained (up to 50 g of DNA).