HiPurA Blood Genomic DNA Midiprep Purification Kit is supplied by HiMedia. HiPurA Genomic DNA Purification Kits provide a fast and easy method for purification of total DNA for reliable applications in PCR, Library screening and Sequencing. The DNA purification procedure using the midiprep columns comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiPure Midiprep Spin Column format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality DNA is obtained. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, ligation, PCR and sequencing. Kit contains:

Resuspension Solution (1X PBS)

Lysis Solution (C1)

Prewash Solution Concentrate (PW)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

HiPure Midiprep Spin Column (in PW144 Collection Tube)

Collection Tube (15 ml conical)

This kit simplifies isolation of DNA from fresh, old (more than 24 hours) and frozen blood with spin-column procedure. Genomic DNA purification from blood involves cell lysis which is achieved by incubation of whole blood in a solution containing chaotropic ions in the presence of Proteinase K at 55C. HiPure Midiprep Spin Column contains specially developed membranes for optimal binding of genomic DNA. After the initial binding of DNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality DNA is finally eluted in the Elution Buffer provided with the kit. Typical yield is 15-50 g of total DNA from 1-2 ml of whole blood.