HiPurATM Blood Genomic DNA Miniprep Purification Kit is supplied by HiMedia. HiPurA Blood Genomic DNA Miniprep Purification Kit provides a fast and easy method for purification of total DNA for downstream applications such as PCR, Southern blotting technique etc. The DNA purification procedure using the miniprep spin column comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality genomic DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR and Southern blotting. Kit contains:

Resuspension Solution (1X PBS)

Lysis Solution (C1)

Prewash Solution Concentrate (PW)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

HiEluteTM Miniprep Spin Column (in PW1139 Collection Tube)

Collection Tubes, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from fresh, old (more than 24 hours) and frozen blood with spin-column procedure. Genomic DNA purification from blood involves cell lysis, which is achieved by incubation of whole blood in a solution containing chaotropic ions in the presence of Proteinase K at 55oC. HiElute Miniprep Spin Column contains specially developed membranes for optimal binding of genomic DNA. After the initial binding of DNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality DNA is finally eluted in the Elution Buffer provided with the kit. Typical yield is 4-12 g of total DNA from 200 l of whole blood.