HiPurA Marine Algal DNA Purification Kit is supplied by HiMedia. DNA extraction from algae remains difficult due to the presence of a rigid cell wall that contains sulphated polysaccharides. Viscous soluble polysaccharides are released by grinding algal material in liquid nitrogen that are difficult to separate from DNA, therefore CTAB method has been applied for DNA extraction. The scale of extraction is dependent on the amount of starting material. This kit contains:

STE Buffer

CTAB Extraction Buffer

RNase A Solution (20 mg/ml)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

HiShredder (in DBCA016 Collection tube)

CTAB (Cetyl trimethylammonium bromide), a detergent is used to break open and solubilize the contents of plant cells. Chlorophyll and some denatured proteins are removed from the plant tissue in an organic chloroform/Isoamyl step and the organic phase is separated by centrifugation. Since the extract contains DNA and RNA, RNA can be removed by the addition of RNase A; the DNA is precipitated and washed in organic solvents before redissolving in aqueous solution. The concentration of the DNA can be estimated by agarose gel electrophoresis. The DNA recovered is suitable for enzyme digestion, Southern blotting and analysis by PCR.