HiPurA Mycobacterium tuberculosis DNA Purification Kit is supplied by HiMedia. HiPurA Mycobacterium tuberculosis DNA Purification Kit provides a fast and easy method for purification of DNA from Mycobacterium spp. for reliable applications in PCR and Southern blotting technique, etc. The DNA purification procedure using the miniprep spin columns comprises of three steps viz, adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR amplification and Southern blotting. This kit contains:

Lysis Solution (AL)

Lysis Solution (C1)

Prewash Solution (PWB)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

Lysozyme

HiBead Tubes

RNase A Solution (20 mg/ml)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from Mycobacterium spp. from bacteria and tissue samples by the spin-column procedure. Bacterial cells are grown in the medium till they reach log phase and are harvested by centrifugation. Samples are subjected to Proteinase K digestion. Following lysis is the binding of DNA to the silica-gel membrane of the HiElute Miniprep Spin column (Capped) to yield approximately upto 20 g of pure DNA. Two rapid wash steps removes trace amount of salt and protein contaminants resulting in the elution of pure DNA in the Elution Buffer provided with the kit.