HiPurA Plant and Fungal RNA Miniprep Purification Kit is supplied by HiMedia. HiPurA Plant and Fungal RNA Miniprep Purification Kit provides a fast and easy method for purification of total RNA for Northern analysis, Poly A+ RNA selection, Primer extension, RNase and S1 nuclease protection assays, RT-PCR, Differential display, Expression-array and expression-chip analysis and cDNA library construction. The RNA purification procedure using the miniprep spin columns comprises of three steps viz, adsorption of RNA to the membrane, removal of residual contaminants and elution of pure RNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high quality RNA is obtained from various species. The RNA obtained is compatible with various downstream applications as mentioned above. This kit contains:

RNA - XPressTM Reagent

Binding Solution (PBR)

Prewash Solution (RW1)

Wash Solution Concentrate (WS)

Elution Solution (RNase- Free Water)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of RNA from plant cells, tissues and filamentous fungi with spincolumn procedure. The RNA - XPress Reagent provided in the kit helps in cell disruption and denaturation of the plant material. After adding Chloroform and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing cell debris and DNA and an organic phase containing proteins. To the aqueous phase, Binding Solution (PBR) and ethanol are added, which promote selective binding of RNA to the HiElute Miniprep Spin Column (Capped) membrane. After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality RNA is finally eluted in the Elution Solution provided with the kit. Typical yield is 15-65 g of total RNA from 100 mg of the plant material, depending on the type of tissue and plant species.