HiPurATM Plant Genomic DNA Miniprep Purification Kit is supplied by HiMedia. HiPurA Plant Genomic DNA Miniprep Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR and Southern blotting technique etc. The DNA purification procedure using the miniprep spin column comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality genomic DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction endonuclease digestion, PCR and Southern blotting. The kit contains:

Lysis Buffer (PL)

Additive-I

Precipitation Buffer (PS)

Binding Buffer Concentrate (BB)

Wash Solution Concentrate (WSP)

Elution Buffer (ET) [10mM Tris-Cl, pH 8.5]

RNase A Solution (20 mg/ml)

HiElute Miniprep Spin Column (Capped) [in DBCA16 Collection Tube]

HiShredder (in DBCA016 Collection tube)

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from fresh plant material with spin column procedure. The procedure is optimized for a maximum of 100 mg of wet-weight of the starting material. The sample (fresh) is cut and ground in liquid nitrogen along with Lysis Buffer (PL). Protein precipitation is followed by removal of other contaminants using HiShredder. The flow-through fraction is then mixed with a solution that enhances the binding of DNA to the column. The solution is then passed through HiElute Miniprep Spin Column (Capped) that is followed by washing steps to remove trace contaminants. High quality DNA is eluted in the Elution Buffer (ET) provided in the kit. Typical yield from 100 mg of wet-weight sample is 5-40 g (depending upon the type of plant used).