HiPurA Plasmid DNA Midiprep Purification Kit is supplied by HiMedia. HiPurA Plasmid DNA Midiprep Purification Kit provides a fast and easy method for purification of total DNA for reliable applications in PCR, library screening and sequencing. The DNA purification procedure using the midiprep spin columns comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure plasmid DNA. HiMedias HiPure Midiprep Spin Column format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality DNA is obtained. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, ligation, PCR and sequencing. This kit contains:

Resuspension Solution (HP1)

Lysis Solution (HP2)

Neutral Solution M (NSM)

Binding Solution (HB)

Wash Solution M (WSM)

Wash Solution Concentrate (HPE)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

RNase A Solution (20 mg/ml)

HiPure Midiprep Spin Column (in PW144 Collection Tube)

HiPure Midiprep Syringe Filter

Collection Tube (15 ml conical)

The harvested bacterial culture is lysed and collected by centrifugation after which it is applied onto the silica column for binding of the DNA molecules in the presence of high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in Elution Buffer. The purified plasmid DNA can be used for immediate use in all molecular biology procedures such as digestion with restriction enzymes, cloning, PCR, transfection, in vitro translation, blotting and sequencing. The kit utilizes an advanced silicabased membrane technology in the form of a convenient spin column, which helps to recover upto 25-100 g of high-copy or low-copy plasmid DNA from upto 150 ml of E. coli culture per isolation procedure.