HiPurA Total RNA Miniprep Purification Kit is supplied by HiMedia. HiPurA Total RNA Miniprep Purification Kit provides a fast and easy method for purification of total RNA for Northern analysis, Poly A+ RNA selection, Primer extension, RNase and S1 nuclease protection assays, RT-PCR, Differential display, Expression-array and expression-chip analysis and cDNA library construction. The RNA purification procedure using the miniprep spin columns comprises of three steps viz, adsorption of RNA to the membrane, removal of residual contaminants and elution of pure RNA. HiMedias HiElute Miniprep Spin column (Capped) format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality RNA is obtained from various species. The RNA obtained is compatible with various downstream applications. This kit contains:

RNA Lysis Solution (HRL)

Prewash Solution (RW1)

Wash Solution Concentrate (WS)

Elution Solution (RNase- Free Water)

HiElute Miniprep Spin Column (Capped) [in DBCA016 Collection Tube]

HiShredder [in DBCA016 Collection Tube]

Collection Tube (Uncapped), Polypropylene (2.0 ml)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of RNA from animal cells, animal tissues and yeast, and for cleanup of RNA from crude enzymatic reactions (e.g. DNase digestion, Proteinase digestion, RNA ligation, and labeling reaction) with spin-column procedure. The lysis buffer provided in the kit helps in cell disruption and denaturation, samples are centrifuged through a HiShredder which removes insoluble material and reduces the viscosity of the lysate by disrupting viscous material. Ethanol is added to the cleared lysate, which promotes selective binding of RNA to the HiElute Miniprep Spin Column (Capped) membrane. After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality RNA is finally eluted in the Elution Solution provided with the kit. A specialized high-salt buffer system allows upto 100 g of total RNA longer than 200 bases to bind to the HiElute Miniprep Spin column (Capped) membrane.