HiPurA Yeast Genomic DNA Purification Kit is supplied by HiMedia. HiPurA Genomic DNA Purification Spin Kits provide a fast and easy method for purification of total DNA for reliable applications in PCR, Southern blotting technique etc. The DNA purification procedure using the miniprep spin columns comprises of three steps viz. adsorption of DNA to the membrane, removal of residual contaminants and elution of pure genomic DNA. HiMedias HiElute Miniprep Spin Column format allows rapid processing of multiple samples. The columns have a high binding capacity and high-quality DNA is obtained from various species. The DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR amplification and Southern blotting. This kit contains:

Lysis Solution (AL)

Lysis Solution (C1)

Prewash Solution (PWB)

Wash Solution Concentrate (WS)

Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]

Proteinase K

RNase A Solution (20 mg/ml)

HiEluteTM Miniprep Spin Column (in PW1139 Collection Tube)

Collection Tube, Polypropylene (2.0 ml)

This kit simplifies isolation of DNA from yeast by the spin-column procedure. Yeast cells (Saccharomyces cerevisiae, Candida albicans), are grown in log phase and spheroplasts are subsequently prepared. Following lysis, the DNA is bound to the silica-gel membrane of the HiElute Miniprep Spin Column. Two rapid wash steps remove trace amount of salt and protein contaminants resulting in the elution of pure DNA in the Elution Buffer provided with the kit.