HiPurATM SPP Blood DNA Isolation Kit is supplied by HiMedia. HiPurA Genomic DNA Purification Kits provide a fast and easy method for isolation and purification of high molecular weight genomic DNA. These kits do not include any time-consuming steps such as CsCl gradient ultracentrifugation and also eliminates the need for phenol-chloroform extraction. These kits also allow rapid processing of multiple samples within 90 minutes. The purified DNA obtained is compatible with downstream applications such as restriction enzyme digestion, PCR and Southern blotting. Kit contains:

RBC Lysis Buffer (10X RBL)

WBC Lysis Buffer (WBL)

Precipitation Buffer (PBP)

Elution Buffer (ET) [10mM Tris-Cl, pH8.5]

RNase A Solution (20 mg/ml)

The HiPurA SPP Blood DNA Isolation Kit simplifies isolation and purification of high molecular weight genomic DNA from fresh, frozen and anticoagulated whole blood by using highly efficient solution-based system. This method can also be used for isolation of genomic DNA from bone marrow, buffy coat and cultured cells. Genomic DNA purification from whole blood involves lysis of red blood cells with RBL buffer (R075) followed by lysis of white blood cells and their nuclei with WBL buffer (DS0046). Impurities like cellular proteins are removed by precipitation and short washing steps while high molecular weight genomic DNA remains in the solution. High quality genomic DNA is then purified by isopropanol precipitation. This system can be scaled up from 0.1 ml to 12 ml of whole blood.