IgG Purification Kit (Protein A Based, Spin Column) is supplied by HiMedia. The IgG Purification Kit (Protein A Based, Spin Column) is based on the affinity of IgG to immobilized Protein A agarose and is used for purification of polyclonal antibodies. When a suspension (e.g. serum, ascites fluid, tissue culture supernatant) containing mixture of substances along with IgG are loaded on the column, the IgG binds to Protein A and is recovered by elution. Kit contains pre-packed ready-to-use columns of 2 ml Protein A agarose resin in 20% ethanol, which has a binding capacity of approximately 25 mg human IgG/ml resin. It also contains:

Protein A Agarose Spin Column (25 Nos)

10X Binding Buffer (100ml)

Elution Buffer (50ml)

Neutralizing Buffer (5ml)

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain of 42 kDa which specifically binds to the Fc region of IgGs. HiMedias Protein A is a recombinant protein, rProtein A, which contains five homologous antibody binding domains with high affinity for subclasses of IgG from many species e.g. human, rabbit, mouse, sheep etc. and for some IgA and IgM. The binding site is located on the Fc region of immunoglobulin. Protein A is covalently coupled to agarose to prepare an affinity matrix for isolating IgGs from various species. It is also used for the purification of IgG fractions (from crude serum, ascites fluid, tissue culture supernatant) and isolation of antigen-antibody complex (during immunoprecipitation). It is advisable to store this kit at 2-8C.