IgG Purification Kit (Protein G Based, Spin Column) is supplied by HiMedia. This kit is designed for simple, rapid antibody purification from serum, ascites and tissue culture supernatant such as those derived from static cultures and bioreactors. This IgG Purification Kit incorporates pre-packed Protein G resin plugs in ready-to-use spin columns. The objective is to offer the researcher total protein purification solutions from the initial fractionation stage to the final polishing steps. The simplicity of Protein G is extremely attractive as it lends itself to the bind, wash and elute mode of operation if the appropriate buffer formulations are selected.

Immunoglobulin G from most species consists of several subclasses with different biological properties. Four subclasses of IgG have been identified in human (IgG1, IgG2, IgG3, and IgG4) and in mouse (IgG1, IgG2a, IgG2b and IgG3). For immunological studies, it is often necessary to isolate one particular subclass of IgG from the other subclasses. Protein G binds to all major Ig classes except IgM and therefore has a wider reactivity profile than Protein A. However, the binding of Igs to Protein G is often stronger, making elution and complete recovery of the immunoglobulin more difficult. Interestingly, due to the lower cost of Protein A compared to Protein G, researchers tend to experiment first with Protein A followed by Protein G.