IgG Purification Kit (Protein L Based, Spin Column) is supplied by HiMedia. Pre-packed Protein L Kit is in a ready-to-use format in which Protein L is immobilized by means of a covalent binding that avoids protein loss and allows for column re-use. This allows column purifications of immunoglobulins containing light chains of type kappa I, III and IV in human and kappa I in mouse classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids.

Protein L is an immunoglobulin-binding protein that was isolated from the bacteria Peptostreptococcus magnus and provides a convenient way to separate immunoglobulins from a variety of sources. Protein L contain four immunoglobulin binding domains of the native protein. Protein L resins may be used for the purification of IgG, IgM, IgA and IgD containing kappa light chains from various species without interfering with the antigen binding site. Besides antibody, Protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv) and domain antibodies (Dabs).