Iscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine, 25mM HEPES Buffer and 3.024gms per liter Sodium bicarbonate, is supplied by HiMedia. It is available in the form of an orangish red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 260.00 -300.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (219.00)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (330.00)

Potassium nitrate (0.07)

Sodium bicarbonate (3204.00)

Sodium chloride (4505.00)

Sodium dihydrogen phosphate anhydrous (109.00)

Sodium selenite (0.017)

Amino acids

Choline chloride (4.00)

D-Biotin (0.01)

D-Ca-Pantothenate (4.00)

Folic acid (4.00)

Nicotinamide (4.00)

Pyridoxal hydrochloride (4.00)

Riboflavin (0.40)

Thiamine hydrochloride (4.00)

Vitamin B12 (0.01)

i-Inositol (7.20)

D-Glucose (4500.00)

HEPES buffer (5958.00)

Phenol red sodium salt (15.00)

Sodium pyruvate (110.00)

Iscove's Modified Dulbecco's Medium (IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. Potassium nitrate is substituted by ferric nitrate. IMDM was the first medium utilizing HEPES buffer. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. This is a 1X Liquid Cell Culture Medium with L-glutamine, 25mM HEPES buffer and sodium bicarbonate. HEPES, a zwitterionic buffer having a pKa of 7.3 at 37C prevents the initial rise in pH that tends to occur at the initiation of a culture and increases the buffering capacity of the medium.