Iscove's Modified Dulbecco's Medium (IMDM) With L-Glutamine and Sodium bicarbonate Without HEPES buffer, is supplied by HiMedia. It is available in the form of a red coloured, clear solution. It has a pH in the range of 7.0 -7.6 and an osmolality between 300.00 -340.00 Osm/Kg H20. It is advisable to store this product between 2-8C and away from bright light. Media composition (in mg/L) is as follows:

Calcium chloride dihydrate (219.00)

Magnesium sulphate anhydrous (97.72)

Potassium chloride (330.00)

Potassium nitrate (0.07)

Sodium bicarbonate (3204.00)

Sodium chloride (4505.00)

Sodium dihydrogen phosphate anhydrous (109.00)

Sodium selenite (0.017)

Amino acids

Choline chloride (4.00)

D-Biotin (0.01)

D-Ca-Pantothenate (4.00)

Folic acid (4.00)

Nicotinamide (4.00)

Pyridoxal hydrochloride (4.00)

Riboflavin (0.40)

Thiamine hydrochloride (4.00)

Vitamin B12 (0.01)

i-Inositol (7.20)

D-Glucose (4500.00)

Phenol red sodium salt (15.00)

Sodium pyruvate (110.00)

Iscove's Modified Dulbecco's Medium (IMDM) is an enriched modification of Dulbecco's Modified Eagle's Medium wherein serum can be partially or totally replaced by chemically defined substances. The medium contains additional amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts. Potassium nitrate is substituted by ferric nitrate. IMDM was the first medium utilizing HEPES buffer. The medium when appropriately supplemented supports good growth of precursor cells of erythrocytes and macrophages. The medium also supports good growth of T and B lymphocytes and a variety of hybrid cells under serum free or reduced serum conditions. This is a 1X Liquid Cell Culture Medium with L-glutamine and sodium bicarbonate. It does not contain HEPES buffer. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.